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Dec 02, 2019· 2.1.1 Place 1 g of fresh or frozen plant tissue in a pre-cooled, sterile and dry mortar and add liquid nitrogen. 2.1.2 Homogenize the plant tissue using a sterile porcelain pestle. 2.1.3 Add 100 mg of the homogenized tissue to a pre-cooled microcentrifuge tube. 2.1.4 Alternatively, 100 μL of plant sap can be used for DNA extraction.

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Microbial biogeography of wine grapes is conditioned by ...

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Aug 05, 2018· The increasing solid residue generation throughout the years, with the civil construction industry being responsible for most of this production, causes serious environmental impacts, since major part of this residue are not discarded appropriately. In this context, the present study intended to analyze the residue management on some public constructions of Barra do Garças City Hall ...

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Lessons from Nature for the Construction of Ceramic ...

The spine of the lance sea urchin (Phyllacanthus imperialis) is an unusual plant-akin hierarchical lightweight construction with several gradation features: a basic core–shell structure is ...

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Impacts of bamboo spreading: a review | SpringerLink

Oct 17, 2019· Richardson et al. define alien plants as plant taxa in a given area whose presence there is due to intentional or accidental introduction as a result of human activity (synonyms: exotic plants, non-native plants; non-indigenous plants). The same authors define invasive plants as naturalized plants that produce reproductive offspring, often in ...

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